Detection of Pathogenic Leptospires in Rat and Shallow Populations and Its Spatial Distribution in Bakaran Kulon Village, Pati District

Background In tropical areas, Leptospirosis is still considered a public health problem, particularly in the event of heavy rainfall and flooding. Transmission leptospira bacteria from infected animals, such as rats, takes place through urine or blood. Different serovar leptospires can be found different reservoir animals field. However, research on presence pathogenic rats Pati Regency, one areas with leptospirosis cases Central Java, very limited. Objectives: The purpose this to identify Leptospira characteristics. Spp rat its distribution Regency. Methods: Rat capture was carried out Bakaran Kulon Village Detection using ropB gene followed by phylogenetic analysis. As additional details, buffer analysis measure distance between positions positive positions. Results: Pathogenic were 11.76% (2/17) shallows area study. Based DNA sequencing, Regency interrogans Bataviae borgpetersenii Ballum, both which are pathogenic. analysis, Leptospira-positive around at distances 30 60 m. Conclusion: This study shows that spp. location cases, indicates have great potential transmitters humans.